Efficient Gel Extraction and PCR Clean-Up 300preps

The Gel/PCR DNA Fragment Extraction Kits are designed to recover or concentrate DNA
fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method
uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments
in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants
are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted
by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can
be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Typically, recoveries are 80-90% for Gel Extraction and 90-95% for PCR Clean-Up. The
entire procedure can be completed in 20 minutes, and the eluted DNA is ready to use in restriction
digestion, ligation, PCR, and sequencing reactions.

KEY FEATURES

SAMPLE SIZE: up to 300mg of agarose gel / up to 100μl of PCR products
FORMAT: Spin Column
OPERATION: Centrifuge / Vacuum Manifold
BINDING CAPACITY: 10μg DNA
EXPECTANT YIELD: 80-90% for gel extraction / 90-95% for PCR Clean-Up
OPERATION TIME: 20 minutes or less
APPLICATIONS: PCR, Fluorescent or Radioactive Sequencing, Restrictive Digestion,
DNA Labeling, Ligation

KIT CONTENTS

Component   IB47030
DF COLUMNS    300
2ML COLLECTION TUBES  300
DF BUFFER 240ml
WASH BUFFER 50ml
(add Ethanol)  (200ml)
ELUTION BUFFER 30ml